Eluents with too high alcohol contents cause an increase in volume of the aqueous phase on the CHROMABOND® XTR. Here the column could be overloaded and the aqueous phase displaced from the column. In this case, a greater capacity column should be used.
Depending on the concentration of the analytes, eluates can be analyzed immediately, or the organic solvent is evaporated. The pH value of the aqueous solution can be altered on the column, which enables elution of different compounds of a sample under optimized conditions. Under certain circumstances, acidic, neutral, and basic compounds can be fractionated in this way.